Transfection of INSL3 gene promoter reporter constructs

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steroids drugs A significant finding is that the major steroid metabolite androstenedione appears to act predominantly as an estrogen and not an androgen in this system. Transfection of INSL3 gene promoter reporter constructs together with various steroid receptor expression plasmids supports these findings and shows that steroid action uses non classical pathways not requiring canonical steroid responsive elements in the proximal promoter region. Together anabolic steroids, the results indicate that increasing estrogens in the follicular phase stimulate a feedforward loop driving INSL3 signaling and thereby promoting steroidogenesis in the growing antral follicle until the LH surge which effectively switches off INSL3 expression.. steroids drugs

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